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Multiplex PCR for Detection of Three Plasmid-Borne Genes of Enteroaggregative Escherichia coli Strains

We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a...

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Main Authors: Cerna, Jorge F., Nataro, James P., Estrada-Garcia, Teresa
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2003
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC154749/
https://ncbi.nlm.nih.gov/pubmed/12734261
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.5.2138-2140.2003
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