Formation of Pseudo-Terminal Restriction Fragments, a PCR-Related Bias Affecting Terminal Restriction Fragment Length Polymorphism Analysis of Microbial Community Structure

Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified genes is a widely used fingerprinting technique in molecular microbial ecology. In this study, we show that besides expected terminal restriction fragments (T-RFs), additional secondary T-RFs occur in T-RFLP analysi...

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Main Authors: Egert, Markus, Friedrich, Michael W.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2003
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Methods
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC154551/
https://ncbi.nlm.nih.gov/pubmed/12732521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.5.2555-2562.2003
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