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Measurement of FRET Efficiency and Ratio of Donor to Acceptor Concentration in Living Cells
Measurement of fluorescence resonance energy transfer (FRET) efficiency and the relative concentration of donor and acceptor fluorophores in living cells using the three-filter cube approach requires the determination of two constants: 1), the ratio of sensitized acceptor emission to donor fluoresce...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1544280/ https://ncbi.nlm.nih.gov/pubmed/16815904 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.088773 |
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