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A continuous fluorimetric assay for protoporphyrinogen oxidase by monitoring porphyrin accumulation
A continuous spectro fluorimetric assay for protoporphyrinogen oxidase (PPO, EC 1.3.3.4) activity has been developed using a 96-well plate reader. Protoporphyrinogen IX, the tetrapyrrole substrate, is a colorless non fluorescent compound. The evolution of the fluorescent tetrapyrrole product, protop...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2005
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1538956/ https://ncbi.nlm.nih.gov/pubmed/16039600 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2005.06.012 |
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