Development of a Real-Time PCR Assay for Quantitative Detection of Encephalitozoon intestinalis DNA

A new real-time PCR assay for quantitation of Encephalitozoon intestinalis DNA was developed which used a TaqMan fluorescent probe for specific detection. Serial dilutions of E. intestinalis spore suspensions obtained from tissue culture were used as external standards. The detection limit of the te...

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Main Authors: Menotti, Jean, Cassinat, Bruno, Sarfati, Claudine, Liguory, Olivier, Derouin, Francis, Molina, Jean-Michel
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2003
Assuntos:
Parasitology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC153894/
https://ncbi.nlm.nih.gov/pubmed/12682122
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.4.1410-1413.2003
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