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Targeting Species-Specific Low-Affinity 16S rRNA Binding Sites by Using Peptide Nucleic Acids for Detection of Legionellae in Biofilms
Using fluorescence in situ hybridization to detect bacterial groups has several inherent limitations. DNA probes are generally used, targeting sites on the 16S rRNA. However, much of the 16S rRNA is highly conserved, with variable regions often located in inaccessible areas where secondary structure...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2006
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1538740/ https://ncbi.nlm.nih.gov/pubmed/16885298 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02918-05 |
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