Quantitative PCR Method for Sensitive Detection of Ruminant Fecal Pollution in Freshwater and Evaluation of This Method in Alpine Karstic Regions

A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively....

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Hauptverfasser: Reischer, Georg H., Kasper, David C., Steinborn, Ralf, Mach, Robert L., Farnleitner, Andreas H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2006
Schlagworte:
Methods
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1538736/
https://ncbi.nlm.nih.gov/pubmed/16885315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00364-06
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