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Random Insertion of a TetR-Inducing Peptide Tag into Escherichia coli Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression

The insertion element InsTipα was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli gene...

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Autori principali: Schlicht, Maximilian, Berens, Christian, Daam, Janko, Hillen, Wolfgang
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1538708/
https://ncbi.nlm.nih.gov/pubmed/16885321
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00739-06
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