Immunological responsiveness of frozen-thawed human lymphocytes.

Mononuclear cells (10--20 X 10(6)) obtained from human peripheral blood by a standard Ficoll-Hypaque technique were suspended in RPMI 1640 media at 4 degrees C containing 10% foetal calf serum and 7-5% dimethyl sulphoxide (DMSO). Two-millilitre aliquots were cooled at -1 degree C/min in a Cryoson BV...

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Detaylı Bibliyografya
Asıl Yazarlar: Strong, D M, Woody, J N, Factor, M A, Ahmed, A, Sell, K W
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1975
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1538319/
https://ncbi.nlm.nih.gov/pubmed/128429
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