Immunological responsiveness of frozen-thawed human lymphocytes.

Mononuclear cells (10--20 X 10(6)) obtained from human peripheral blood by a standard Ficoll-Hypaque technique were suspended in RPMI 1640 media at 4 degrees C containing 10% foetal calf serum and 7-5% dimethyl sulphoxide (DMSO). Two-millilitre aliquots were cooled at -1 degree C/min in a Cryoson BV...

Description complète

Enregistré dans:
Détails bibliographiques
Auteurs principaux: Strong, D M, Woody, J N, Factor, M A, Ahmed, A, Sell, K W
Format: Artigo
Langue:Inglês
Publié: 1975
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1538319/
https://ncbi.nlm.nih.gov/pubmed/128429
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!

Documents similaires