Construction of a Self-Excisable Bacterial Artificial Chromosome Containing the Human Cytomegalovirus Genome and Mutagenesis of the Diploid TRL/IRL13 Gene

The full-length genome of human cytomegalovirus strain AD169 was cloned as an infectious bacterial artificial chromosome (BAC) plasmid, pAD/Cre. The BAC vector, flanked by LoxP sites, was inserted immediately after the Us28 open reading frame without deletion of any viral sequences. The BAC vector c...

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Autors principals: Yu, Dong, Smith, Gregory A., Enquist, Lynn W., Shenk, Thomas
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2002
Matèries:
Replication
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC153828/
https://ncbi.nlm.nih.gov/pubmed/11836410
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