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Antibodies to human glomerular basement membrane: modified methodology for detection in human serum.

A new radioimmunoassay for detection of circulating antibodies to human glomerular basement membrane (GBM) is described. GBM was isolated, purified, and an antigenic component extracted by 8 M urea incubation. The 8 M urea extract was radiolabelled, separated on Sephadex G-150, and employed as GBM a...

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Detalhes bibliográficos
Main Authors: Buffaloe, G W, Evans, J E, McIntosh, R M, Glassock, R J, Tavel, T B, Terman, D S
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1537934/
https://ncbi.nlm.nih.gov/pubmed/7389188
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