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A recombinant topoisomerase I used for autoantibody detection in sera from patients with systemic sclerosis.

We report the expression of a cDNA clone encoding 695 carboxyl-terminal amino acids of human DNA topoisomerase I (topoI) in Escherichia coli. More than 96% of the anti-HeLa topoI-positive sera from patients with a connective tissue disease displayed also an immunoreactivity with this recombinant pro...

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Detalhes bibliográficos
Main Authors: Verheijen, R, Van den Hoogen, F, Beijer, R, Richter, A, Penner, E, Habets, W J, van Venrooij, W J
Formato: Artigo
Idioma:Inglês
Publicado em: 1990
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1535233/
https://ncbi.nlm.nih.gov/pubmed/1691063
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