Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance

BACKGROUND: A limiting factor of cDNA microarray technology is the need for a substantial amount of RNA per labeling reaction. Thus, 20–200 micro-grams total RNA or 0.5–2 micro-grams poly (A) RNA is typically required for monitoring gene expression. In addition, gene expression profiles from large,...

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Detalhes bibliográficos
Main Authors: Nygaard, Vigdis, Løland, Anders, Holden, Marit, Langaas, Mette, Rue, Håvard, Liu, Fang, Myklebost, Ola, Fodstad, Øystein, Hovig, Eivind, Smith-Sørensen, Birgitte
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2003
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC153514/
https://ncbi.nlm.nih.gov/pubmed/12659661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-4-11
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