Development and Evaluation of a Quantitative, Touch-Down, Real-Time PCR Assay for Diagnosing Pneumocystis carinii Pneumonia

A rapid (time to completion, <4 h, including DNA extraction) and quantitative touch-down (QTD) real-time diagnostic Pneumocystis carinii PCR assay with an associated internal control was developed, using fluorescence resonance energy transfer (FRET) probes for detection. The touch-down procedure sig...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Larsen, Hans Henrik, Masur, Henry, Kovacs, Joseph A., Gill, Vee J., Silcott, Victoria A., Kogulan, Palaniandy, Maenza, Janine, Smith, Margo, Lucey, Daniel R., Fischer, Steven H.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2002
Sujets:	Mycology
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC153364/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11825961/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.2.490-494.2002
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