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A GFP-based System to Uncouple mRNA Transport from Translation in a Single Living Neuron

An inducible fluorescent system based on GFP is presented that allows for the uncoupling of dendritic mRNA transport from subsequent protein synthesis at the single cell level. The iron-responsive element (IRE) derived from ferritin mRNA in the 5′-UTR of the GFP reporter mRNA renders translation of...

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Bibliografische gegevens
Hoofdauteurs: Macchi, Paolo, Hemraj, Indradeo, Goetze, Bernhard, Grunewald, Barbara, Mallardo, Massimo, Kiebler, Michael A.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The American Society for Cell Biology 2003
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC153123/
https://ncbi.nlm.nih.gov/pubmed/12686610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E02-08-0505
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