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Synthesis of GDP-Mannose and Mannosylglycerate from Labeled Mannose by Genetically Engineered Escherichia coli without Loss of Specific Isotopic Enrichment

We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-α-d-mannosyl-d-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, en...

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Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Sampaio, Maria-Manuel, Santos, Helena, Boos, Winfried
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 2003
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Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC152461/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12514000/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.1.233-240.2003
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