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Optimal Growth and Ethanol Production from Xylose by Recombinant Saccharomyces cerevisiae Require Moderate d-Xylulokinase Activity

d-Xylulokinase (XK) is essential for the metabolism of d-xylose in yeasts. However, overexpression of genes for XK, such as the Pichia stipitis XYL3 gene and the Saccharomyces cerevisiae XKS gene, can inhibit growth of S. cerevisiae on xylose. We varied the copy number and promoter strength of XYL3...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Jin, Yong-Su, Ni, Haiying, Laplaza, Jose M., Jeffries, Thomas W.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2003
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC152454/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12514033/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.1.495-503.2003
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