Gene Cloning, Purification, and Characterization of a Phosphodiesterase from Delftia acidovorans
A novel phosphodiesterase (PdeA) was purified from Delftia acidovorans, the gene encoding the enzyme was cloned and expressed in Escherichia coli, and the recombinant enzyme was purified to apparent homogeneity and characterized. PdeA is an 85-kDa trimer that exhibits maximal activity at 65°C and pH...
Guardat en:
| Publicat a: | Appl Environ Microbiol |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
2003
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC152426/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12514034/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.1.504-508.2003 |
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