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Gene Cloning, Purification, and Characterization of a Phosphodiesterase from Delftia acidovorans

A novel phosphodiesterase (PdeA) was purified from Delftia acidovorans, the gene encoding the enzyme was cloned and expressed in Escherichia coli, and the recombinant enzyme was purified to apparent homogeneity and characterized. PdeA is an 85-kDa trimer that exhibits maximal activity at 65°C and pH...

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Библиографические подробности
Главные авторы: Tehara, Sundiep K., Keasling, Jay D.
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Microbiology 2003
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC152426/
https://ncbi.nlm.nih.gov/pubmed/12514034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.1.504-508.2003
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