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Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution
Aldolases have potential as tools for the synthesis of stereochemically complex carbohydrates. Here, we show that directed evolution can be used to alter the stereochemical course of the reaction catalyzed by tagatose-1,6-bisphosphate aldolase. After three rounds of DNA shuffling and screening, the...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC152260/ https://ncbi.nlm.nih.gov/pubmed/12626743 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0635924100 |
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