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A high-throughput gene expression analysis technique using competitive PCR and matrix-assisted laser desorption ionization time-of-flight MS

We report here an approach for gene expression analysis by combining competitive PCR and matrix-assisted laser desorption ionization time-of-flight MS. A DNA standard is designed with an artificial single nucleotide polymorphism in the gene of interest. The standard is added to the reverse transcrip...

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Détails bibliographiques
Auteurs principaux: Ding, Chunming, Cantor, Charles R.
Format: Artigo
Langue:Inglês
Publié: The National Academy of Sciences 2003
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC152246/
https://ncbi.nlm.nih.gov/pubmed/12624187
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0630494100
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