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A bioluminescence resonance energy transfer (BRET) system: Application to interacting circadian clock proteins

We describe a method for assaying protein interactions that offers some attractive advantages over previous assays. This method, called bioluminescence resonance energy transfer (BRET), uses a bioluminescent luciferase that is genetically fused to one candidate protein, and a green fluorescent prote...

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Autors principals: Xu, Yao, Piston, David W., Johnson, Carl Hirschie
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC15108/
https://ncbi.nlm.nih.gov/pubmed/9874787
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