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Reproducibility of oligonucleotide arrays using small samples

BACKGROUND: Low RNA yields from small tissue samples can limit the use of oligonucleotide microarrays (Affymetrix GeneChips(®)). Methods using less cRNA for hybridization or amplifying the cRNA have been reported to reduce the number of transcripts detected, but the effect on realistic experiments d...

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Detalhes bibliográficos
Main Authors: McClintick, Jeanette N, Jerome, Ronald E, Nicholson, Charles R, Crabb, David W, Edenberg, Howard J
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC150597/
https://ncbi.nlm.nih.gov/pubmed/12594857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-4-4
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