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Identification of a New Vertebrate Nucleoporin, Nup188, with the Use of a Novel Organelle Trap Assay

The study of the nuclear pore in vertebrates would benefit from a strategy to directly identify new nucleoporins and interactions between those nucleoporins. We have developed a novel two-step “organelle trap” assay involving affinity selection and in vitro pore assembly. In the first step, soluble...

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Bibliografiske detaljer
Udgivet i:Mol Biol Cell
Principais autores: Miller, Brian R., Powers, Maureen, Park, Minkyu, Fischer, Wolfgang, Forbes, Douglass J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Cell Biology 2000
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15000/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11029043/
https://ncbi.nlm.nih.govhttps://doi.org/10.1091/mbc.11.10.3381
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