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Evaluation of Semiautomated Multiplex PCR Assay for Determination of Streptococcus pneumoniae Serotypes and Serogroups

A semiautomated method for the determination of five serotypes and three serogroups in Streptococcus pneumoniae was developed. Primers specific for serotypes 1, 3, 14, 19F, and 23F and serogroups 6, 19, and 23 were combined in three multiplex PCRs. Products were separated by capillary electrophoresi...

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Detalhes bibliográficos
Main Authors: Lawrence, Elliot R., Griffiths, David B., Martin, Siobhán A., George, Robert C., Hall, Lucinda M. C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC149661/
https://ncbi.nlm.nih.gov/pubmed/12574253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.2.601-607.2003
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