Nested PCR Assays for Detection of Blastomyces dermatitidis DNA in Paraffin-Embedded Canine Tissue

A Blastomyces dermatitidis nested PCR assay targeting the gene encoding the Wisconsin 1 (WI-1) adhesin was developed and compared with a nested PCR targeting the 18S rRNA gene (rDNA) of members of the family Onygenaceae. We examined 73 paraffin-embedded tissue samples obtained from nine dogs which d...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Bialek, Ralf, Cascante Cirera, Anna, Herrmann, Tanja, Aepinus, Christian, Shearn-Bochsler, Valerie I., Legendre, Alfred M.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Mycology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC149559/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12517849/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.1.205-208.2003
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