Generation of an Infectious Clone of VR-2332, a Highly Virulent North American-Type Isolate of Porcine Reproductive and Respiratory Syndrome Virus

A full-length cDNA clone of the prototypical North American porcine reproductive and respiratory syndrome virus (PRRSV) isolate VR-2332 was assembled in the plasmid vector pOK(12). To rescue infectious virus, capped RNA was transcribed in vitro from the pOK(12) clone and transfected into BHK-21C cel...

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Detalhes bibliográficos
Main Authors: Nielsen, H. S., Liu, G., Nielsen, J., Oleksiewicz, M. B., Bøtner, A., Storgaard, T., Faaberg, K. S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Replication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC149535/
https://ncbi.nlm.nih.gov/pubmed/12610145
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.77.6.3702-3711.2003
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