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TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening

Site-directed mutagenesis is an invaluable tool for functional studies and genetic engineering. However, most current protocols require the target DNA to be cloned into a plasmid vector before mutagenesis can be performed, and none of them are effective for multiple-site mutagenesis. We now describe...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Young, Lei, Dong, Qihan
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2003
Soggetti:
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC149223/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12560514/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng01
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