Internally Controlled Real-Time PCR Method for Quantitative Species-Specific Detection and vapA Genotyping of Rhodococcus equi

We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE...

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Bibliografiske detaljer
Main Authors: Rodríguez-Lázaro, David, Lewis, Deborah A., Ocampo-Sosa, Alain A., Fogarty, Ursula, Makrai, László, Navas, Jesús, Scortti, Mariela, Hernández, Marta, Vázquez-Boland, José A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2006
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Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1489618/
https://ncbi.nlm.nih.gov/pubmed/16751540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02706-05
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