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Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish Samples

A standardized real-time reverse transcription-PCR (RT-PCR) assay has been developed for an accurate estimation of the number of genome copies of hepatitis A virus (HAV) in clinical and shellfish samples. Real-time procedures were based on the amplification of a fragment of the highly conserved 5′ n...

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Hlavní autoři: Costafreda, M. Isabel, Bosch, Albert, Pintó, Rosa M.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1489592/
https://ncbi.nlm.nih.gov/pubmed/16751488
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02660-05
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