Phage Display of an Intracellular Carboxylesterase of Bacillus subtilis: Comparison of Sec and Tat Pathway Export Capabilities

Using the phage display technology, a protein can be displayed at the surface of bacteriophages as a fusion to one of the phage coat proteins. Here we describe development of this method for fusion of an intracellular carboxylesterase of Bacillus subtilis to the phage minor coat protein g3p. The car...

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Библиографические подробности
Главные авторы: Dröge, Melloney J., Boersma, Ykelien L., Braun, Peter G., Buining, Robbert Jan, Julsing, Mattijs K., Selles, Karin G. A., van Dijl, Jan Maarten, Quax, Wim J.
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Microbiology 2006
Предметы:
Enzymology and Protein Engineering
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC1489362/
https://ncbi.nlm.nih.gov/pubmed/16820448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02750-05
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