Phage Display of an Intracellular Carboxylesterase of Bacillus subtilis: Comparison of Sec and Tat Pathway Export Capabilities

Using the phage display technology, a protein can be displayed at the surface of bacteriophages as a fusion to one of the phage coat proteins. Here we describe development of this method for fusion of an intracellular carboxylesterase of Bacillus subtilis to the phage minor coat protein g3p. The car...

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Detalhes bibliográficos
Main Authors: Dröge, Melloney J., Boersma, Ykelien L., Braun, Peter G., Buining, Robbert Jan, Julsing, Mattijs K., Selles, Karin G. A., van Dijl, Jan Maarten, Quax, Wim J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1489362/
https://ncbi.nlm.nih.gov/pubmed/16820448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02750-05
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