Purification and Characterization of Two Enantioselective α-Ketoglutarate-Dependent Dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH

α-Ketoglutarate-dependent (R)-dichlorprop dioxygenase (RdpA) and α-ketoglutarate-dependent (S)-dichlorprop dioxygenase (SdpA), which are involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH, were expressed and purified as His(6)-tagged fusion proteins fro...

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Main Authors: Müller, Tina A., Fleischmann, Thomas, van der Meer, Jan Roelof, Kohler, Hans-Peter E.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2006
Assuntos:
Enzymology and Protein Engineering
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1489315/
https://ncbi.nlm.nih.gov/pubmed/16820480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02758-05
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