Selected classes of minimised hammerhead ribozyme have very high cleavage rates at low Mg2+ concentration.
In vitro selection was used to enrich for highly efficient RNA phosphodiesterases within a size-constrained (18 nt) ribonucleotide domain. The starting population (g0) was directed in trans against an RNA oligonucleotide substrate immobilised to an avidin-magnetic phase. Four rounds of selection wer...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1999
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148808/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10325431/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.11.2400 |
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