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In situ localized amplification and contact replication of many individual DNA molecules.

We describe a method to clone and amplify DNA by performing the polymerase chain reaction (PCR) in a thin polyacrylamide film poured on a glass microscope slide. The polyacrylamide matrix retards the diffusion of the linear DNA molecules so that the amplification products remain localized near their...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Mitra, R D, Church, G M
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1999
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC148757/
https://ncbi.nlm.nih.gov/pubmed/10572186
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