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Direct sequencing of RAPD fragments using 3'-extended oligonucleotide primers and dye terminator cycle-sequencing.

Random amplified polymorphic DNA (RAPD) markers are used widely to develop high resolution genetic maps and for genome fingerprinting. Typically, single oligomers of approximately 10 nucleotides are used to PCR amplify characteristic RAPD marker fragments. We describe an efficient method for the dir...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Autores principales: Mitchelson, K R, Drenth, J, Duong, H, Chaparro, J X
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 1999
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148638/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10481040/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.19.e28
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