Isolation of altered specificity mutants of the single-chain 434 repressor that recognize asymmetric DNA sequences containing the TTAA and TTAC subsites.

A novel single-chain (sc) protein framework containing covalently dimerized DNA-binding domains (DBD) of the phage 434 repressor was used to construct combinatorial mutant libraries in order to isolate mutant DBDs with altered specificities. The library members contain one wild-type DBD and one muta...

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Détails bibliographiques
Auteurs principaux: Simoncsits, A, Tjörnhammar, M L, Wang, S, Pongor, S
Format: Artigo
Langue:Inglês
Publié: 1999
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC148589/
https://ncbi.nlm.nih.gov/pubmed/10446235
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