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Facile characterization of translation initiation via nonsense codon suppression.

A new strategy for studying the mechanism of translation initiation in eukaryotes has been developed. The strategy involves the use of an in vitro translation system to incorporate a non-natural fluorescent amino acid into a protein from a suppressor tRNAPheCUA misacylated with that amino acid. It i...

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Bibliografiske detaljer
Main Authors: Karginov, A V, Lodder, M, Hecht, S M
Format: Artigo
Sprog:Inglês
Udgivet: 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC148561/
https://ncbi.nlm.nih.gov/pubmed/10454635
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