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Pyrophosphorolytic dismutation of oligodeoxy-nucleotides by terminal deoxynucleotidyltransferase.

Terminal transferase (TdT), when incubated with a purified(32)P-5"-end-labeled oligonucleotide of defined length in the presence of Co(2+), Mn(2+)or Mg(2+)and 2-mercaptoethanol in cacodylate or HEPES buffer, pH 7.2, exhibits the ability to remove a 3"-nucleotide from one oligonucleotide an...

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Bibliografiske detaljer
Main Authors: Anderson, R S, Bollum, F J, Beattie, K L
Format: Artigo
Sprog:Inglês
Udgivet: 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC148547/
https://ncbi.nlm.nih.gov/pubmed/10454617
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