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Identification of differentially expressed 5'-end mRNA variants by an improved RACE technique (PEETA).

A rapid and efficient procedure is described for mapping and cloning the 5'-ends of mRNAs, including those generated from a unique gene by alternative splicing and promoter usage. This method involves reverse transcription of the targeted mRNAs from a long, highly labeled specific primer, resol...

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Detalhes bibliográficos
Main Authors: Flouriot, G, Brand, H, Gannon, F
Formato: Artigo
Idioma:Inglês
Publicado em: 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC148519/
https://ncbi.nlm.nih.gov/pubmed/10454627
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