Prevention of depurination during elution facilitates the reamplification of DNA from differential display gels.
DNA fragments that show a pattern of differential expression on differential display gels must be eluted from the gel matrix and reamplified to enable further analysis. Elution is usually achieved by heating excised gel slices in a small volume of either water or TE. Here we show that this elution s...
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| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1999
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148517/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10454625/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.15.e6 |
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