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Identification of genes regulated by muscarinic acetylcholine receptors: application of an improved and statistically comprehensive mRNA differential display technique.

In order to identify genes that are regulated by muscarinic acetylcholine receptors, we developed an mRNA differential display technique (DD) approach. By increasing redundancy and by evaluating optimised reagents and conditions for reverse transcription of total RNA, PCR and separation of PCR produ...

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Autors principals: von der Kammer, H, Albrecht, C, Mayhaus, M, Hoffmann, B, Stanke, G, Nitsch, R M
Format: Artigo
Idioma:Inglês
Publicat: 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC148442/
https://ncbi.nlm.nih.gov/pubmed/10219095
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