DNA hybridization on microparticles: determining capture-probe density and equilibrium dissociation constants.

Many DNA-probe assays utilize oligonucleotide-coated microparticles for capture of complementary nucleic acids from solution. During development of these assays, as well as in other particle-based nucleic acid applications, it is useful to know both the amount of duplex formation expected under vari...

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Main Authors: Wilkins Stevens, P, Henry, M R, Kelso, D M
Format: Artigo
Sprog:Inglês
Udgivet: 1999
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC148376/
https://ncbi.nlm.nih.gov/pubmed/10076004
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