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Transplacement mutagenesis: a novel in situ mutagenesis system using phage-plasmid recombination.

Site-specific mutagenesis provides the ability to alter DNA with precision so that the function of any given gene can be more fully understood. Several methods of in vitro mutagenesis are time-consuming and imprecise, requiring the subcloning and sequencing of products. Here we describe a rapid, hig...

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Autors principals: Unger, M W, Liu, S Y, Rancourt, D E
Format: Artigo
Idioma:Inglês
Publicat: 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC148341/
https://ncbi.nlm.nih.gov/pubmed/10037809
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