Random mutagenesis by recombinational capture of PCR products in Bacillus subtilis and Acinetobacter calcoaceticus.

We describe a general method for random mutagenesis of cloned genes by error-prone PCR or DNA shuffling that eliminates the need for post-amplification subcloning following each cycle of mutagenesis. This method exploits the highly efficient and recombinogenic nature of DNA uptake during natural tra...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Melnikov, A, Youngman, P J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1999
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148286/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9927739/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.4.1056
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