Calcium dependence of aequorin bioluminescence dissected by random mutagenesis

Aequorin bioluminescence is emitted as a rapidly decaying flash upon calcium binding. Random mutagenesis and functional screening were used to isolate aequorin mutants showing slow decay rate of luminescence. Calcium sensitivity curves were shifted in all mutants, and an intrinsic link between calci...

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Bibliographic Details
Main Authors: Tricoire, Ludovic, Tsuzuki, Keisuke, Courjean, Olivier, Gibelin, Nathalie, Bourout, Gaëlle, Rossier, Jean, Lambolez, Bertrand
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2006
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1480436/
https://ncbi.nlm.nih.gov/pubmed/16769886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0603176103
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