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Simultaneous detection of several oligonucleotides by time-resolved fluorometry: the use of a mixture of categorized microparticles in a sandwich type mixed-phase hybridization assay.

Porous, uniformly sized (50 micrometer) glycidyl methacrylate/ethylene dimethacrylate particles (SINTEF) were used as a solid phase to construct a sandwich type hybridization assay that allowed simultaneous detection of up to six oligonucleotides from a single sample. The assay was based on categori...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Hakala, H, Virta, P, Salo, H, Lönnberg, H
Format: Artigo
Language:Inglês
Published: Oxford University Press 1998
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148034/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9837986/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.24.5581
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