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A Schizosaccharomyces pombe artificial chromosome large DNA cloning system.

The feasibility of using the fission yeast, Schizosaccharomyces pombe , as a host for the propagation of cloned large fragments of human DNA has been investigated. Two acentric vector arms were utilized; these carry autonomously replicating sequences ( ars elements), selectable markers ( ura4(+) or...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Young, D J, Nimmo, E R, Allshire, R C
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1998
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147965/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9801299/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.22.5052
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