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A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination

BACKGROUND: Cre-loxP recombination refers to the process of site-specific recombination mediated by two loxP sequences and the Cre recombinase protein. Transgenic experiments exploit integrative recombination, where a donor plasmid carrying a loxP site and DNA of interest integrate into a recipient...

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Detalhes bibliográficos
Main Authors: Missirlis, Perseus I, Smailus, Duane E, Holt, Robert A
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1479339/
https://ncbi.nlm.nih.gov/pubmed/16595017
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-7-73
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