Multiplex PCR-Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes

We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 “unknowns.” The assay identified all target serotypes, but 11, which cross-react w...

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Bibliographic Details
Main Authors: Kong, Fanrong, Brown, Mitchell, Sabananthan, Archcna, Zeng, Xianyu, Gilbert, Gwendolyn L.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2006
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1479170/
https://ncbi.nlm.nih.gov/pubmed/16672432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.44.5.1887-1891.2006
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