Primer extension analysis of eukaryotic ribosome-mRNA complexes.
Conditions are described for using a primer extension inhibition (toeprinting) assay to study the initiation step of protein synthesis in rabbit reticulocyte lysates. These studies revealed that chloramphenicol acetyltransferase mRNA, which is widely used as a reporter, forms unusually labile initia...
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| Publicado en: | Nucleic Acids Res |
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| Autor principal: | |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1998
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147915/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9776744/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.21.4853 |
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