Use of gap repair in fission yeast to obtain novel alleles of specific genes.
We have adapted a method for making libraries of mutations in any specific gene for use in the fission yeast Schizosaccharomyces pombe . This elegant and simple method consists of PCR amplification of the gene of interest, followed by co-transformation of fission yeast with the PCR fragment and a li...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1998
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147907/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9753750/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.20.4783 |
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